The Hippo signaling pathway controls the regulation of organ growth and development. Dysfunctions of this pathway are related to various types of cancers, making it a critical area of study for cancer research and the development of regenerative medicines. This study investigates the manipulation of gene Minibrain (mnb). Mnb is a kinase that phosphorylates other proteins. Understanding the function of Mnb will ultimately allow us to understand its role within the Hippo pathway. Mnb is thought to affect the function and localization of the kinase Warts (Wts), which determines Yorkie (Yki) activation and consequently the outcomes of the Hippo pathway. (Fig. 3) We hypothesize that high levels of mnb will cause overgrowth and affect Wts localization, and vice versa. We employed gene manipulation techniques using the UAS/Gal 4 system (Fig. 1). Using immunofluorescence and confocal microscopy, we analyzed the effects of different levels of mnb on Yki. Additionally, we assessed the phenotypic consequences of the varying levels of mnb on wing growth. These findings will guide further exploration of the Hippo signaling pathway and its broader implications.
